Chemical modifications in P450 systems

Last modified: Thu Apr 15 13:41:56 BST 2004

• Adamovich, T.B., Pikuleva, I.A., Chashchin, V.L. and Usanov, S.A. (1989) Selective chemical modification of cytochrome P-450SCC lysine residues. Identification of lysines involved in the interaction with adrenodoxin. Biochim. Biophys. Acta 996, 247-253.
• Chashchin, V.L., Turko, I.V., Akhrem, A.A. and Usanov, S.A. (1985) Cross-linking studies of adrenocortical cytochrome P-450scc. Evidence for a covalent complex with adrenodoxin and localization of the adrenodoxin-binding domain. Biochim. Biophys. Acta 828, 313-324.
• Cvrk, T., Hodek, P. and Strobel, H.W. (1996) Identification and characterization of cytochrome P4501A1 amino acid residues interacting with a radiolabeled photoaffinity diazido-benzphetamine analogue. Arch. Biochem. Biophys. 330, 142-152.
• Golly, I. and Hlavica, P. (1992) Chemical modification of lysine residues in cytochrome P450LM2 (P450IIB4): influence on heme liganding of arylamines. Arch. Biochem. Biophys. 292, 287-294.
• Golly, I. and Hlavica, P. (1993) Inactivation of phenobarbital-inducible rabbit-liver microsomal cytochrome P-450 by allylisopropylacetamide: impact on electron transfer. Biochim. Biophys. Acta 1142, 74-82.
• Guryev, O., Dubrovsky, T., Chernogolov, A., Dubrovskaya, S., Usanov, S. and Nicolini, C. (1997) Orientation of cytochrome P450scc in Langmuir-Blodgett monolayers. Langmuir 13, 299-304.
• Janig, G.R., Dettmer, R., Usanov, S.A. and Ruckpaul, K. (1983) Identification of the ligand trans to thiolate in cytochrome P-450 LM2 by chemical modification. FEBS Lett. 159, 58-62.
• Koenigs, L.L. and Trager, W.F. (1998) Mechanism-based inactivation of P450 2A6 by furanocoumarins. Biochemistry 37, 10047-10061.
• Kunz, B.C. and Richter, C. (1983) Chemical modification of microsomal cytochrome P450: role of lysyl residues in hydroxylation activity. FEBS Lett. 161, 311-314.
• Pikuleva, I.A., Lapko, A.G. and Chashchin, V.L. (1992) Functional reconstitution of cytochrome P-450scc with hemin activated with Woodward's reagent K. Formation of a hemeprotein cross-link. J. Biol. Chem. 267, 1438-1442.
• Roberts, E.S., Pernecky, S.J., Alworth, W.L. and Hollenberg, P.F. (1996) A role for threonine 302 in the mechanism-based inactivation of P450 2B4 by 2-ethynylnaphthalene. Arch. Biochem. Biophys. 331, 170-176.
• Shen, S. and Strobel, H.W. (1993) Role of lysine and arginine residues of cytochrome P450 in the interaction between cytochrome P4502B1 and NADPH-cytochrome P450 reductase. Arch. Biochem. Biophys. 304, 257-265.
• Taniguchi, T. and Kimura, T. (1976) Studies on nitrotyrosine-82 and aminotyrosine-82 derivatives of adrenodoxin. Effects of chemical modification on the complex formation with adrenodoxin reductase. Biochemistry 15, 2849-2853.
• Tew, D.G. (1993) Inhibition of cytochrome P450 reductase by the diphenyliodonium cation. Kinetic analysis and covalent modifications. Biochemistry 32, 10209-10215.
• Tsubaki, M., Tomita, S., Tsuneoka, Y. and Ichikawa, Y. (1986) Characterization of two cysteine residues in cytochrome P-450scc: chemical identification of the heme-binding cysteine residue. Biochim. Biophys. Acta 870, 564-574.
• Turko, I.V., Adamovich, T.B., Kirillova, N.M., Usanov, S.A. and Chashchin, V.L. (1989) Cross-linking studies of the cholesterol hydroxylation system from bovine adrenocortical mitochondria. Biochim. Biophys. Acta 996, 37-42.
• Usanov, S.A., Pikuleva, I.A., Chashchin, V.L. and Akhrem, A.A. (1992) Chemical modification of adrenocortical cytochrome P-450scc with tetranitromethane. Biochim. Biophys. Acta 790, 259-267.
• Yun, C.H., Hammons, G.J., Jones, G., Martin, M.V., Hopkins, N.E., Alworth, W.L. and Guengerich, F.P. (1992) Modification of cytochrome P450 1A2 enzymes by the mechanism-based inactivator 2-ethynylnaphthalene and the photoaffinity label 4-azidobiphenyl. Biochemistry 31, 10556-10563.

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