Microscopy 2018

 

 

FluoMicro@ICGEB is organized in morning theoretical sessions on live imaging and afternoon practical sessions on the instruments. Cutting-edge talks from international experts will complement the practical course with an overview of the recent advances in the field.

 

Participants will be divided in groups of maximum 10 members who will rotate between the four practical stations in the afternoon:

-       Station 1: Confocal imaging – Zeiss LSM880 with Airyscan module

Alessandro Cometta

www.icgeb.org/fluorescence-microscopy.html

-       Station 2: Live imaging – Nikon Eclipse Ti Inverted Microscope with Okolab stage incubator

Paola Massimi and Tea Carletti

www.icgeb.org/fluorescence-microscopy.html

-       Station 3: High-throughput Screening - Molecular Devices ImageXpress or PerkinElmer Operetta high-content screening

-       Luca Braga and Gianluca Pegoraro

www.icgeb.org/high-throughput-screening.html

-       Station 4: Image processing and quantitative analysis – ImageJ/Fiji freeware

Paolo Maiuri and Davide Mazza

FluoMicro@ICGEB is located within the premises of the ICGEB located in the AREA Science Park – Padriciano, 99 – Trieste.

 

Preliminary Programme

 

Tuesday 2nd October 2018

08:00

pick-up from Hotel

 
08.30

Course Registration

ICGEB Foyer, ‘W’ Building
09.15 Welcome address

Alessandro Marcello, ICGEB Trieste, Italy

09.30

Fluorescence Microscopy: from basic principles to instrumental options

Gabriele Baj, University of Trieste, Italy

10.30

Setup and technology of a confocal microscope

Alessandro Cometta, Zeiss Milano, Italy
11.30

Coffee break

ICGEB Foyer, ‘W’ Building
12.00

Cutting-edge Technology: Liquid Tunable Microscopy: a new paradigm in Optical Microscopy

Alberto Diaspro, IIT, Genova, Italy

13.30

Lunch

Area Science Park cafeteria, ground floor, 'C' Building

14.30

 

 

Practical session I (station 1, 2, 3 & 4)

 

 

Paola Massimi and Luca Braga, ICGEB Trieste, Italy
Alessandro Cometta
Paolo Maiuri, IFOM, Milan, Italy
Davide Mazza, San Raffaele, Milan, Italy

16.45

Practical session II (station 1, 2, 3 & 4)

Paola Massimi, Luca Braga, Alessandro Cometta, Paolo Maiuri and Davide Mazza
19.00

Wine & Cheese

ICGEB Foyer, ‘W’ Building
     

Wednesday 3rd October 2018

09.00

High-throughput imaging

Gianluca Pegoraro, NIH, Bethesda, USA
10.00

Acquisition and processing of images

Davide Mazza
11.00

Coffee break

ICGEB Foyer, ‘W’ Building
11.30

Optimization of specimens for fluorescence microscopy

Tea Carletti, ICGEB Trieste, Italy

12.30

Cutting-edge Technology: Tracking Golgi derived vesicles during phagocytosis in macrophages

Dhiraj Kumar, ICGEB New Delhi, India

13.30

Lunch

Area Science Park cafeteria, ground floor, 'C' Building
14.30

Practical session I (station 1, 2, 3 & 4)

Paola Massimi, Luca Braga, Alessandro Cometta, Paolo Maiuri and Davide Mazza
16.45

Practical session II (station 1, 2, 3 & 4)

Paola Massimi, Luca Braga, Alessandro Cometta, Paolo Maiuri and Davide Mazza
20.00

Happy hour

 
     

Thursday 4th October 2017

09.00

Cutting-edge Technology: use of TIR-FM to unveil the mechanobiology of the cell

Nils Gauthier, IFOM, Milan, Italy

10.00

Cutting-edge Technology: CLEM: Bridging the light and electron microscopy

Elena Polishchuk, TIGEM, Naples, Italy

11.00

Coffee break

ICGEB Foyer, ‘W’ Building
11.30

Cutting-edge Technology: Tracking vesicular trafficking in immune cells

Federica Benvenuti, ICGEB Trieste, Italy

12.30

Cutting-edge Technology: Polarity transmission: from the cytoskeleton to the nucleus

Paolo Maiuri

13.15

Concluding remarks, attendance certificates

 
13.30 Lunch Area Science Park cafeteria, ground floor, 'C' Building
     
Afternoon session

14.30-16.30

 Demonstration of a low-cost, open-source super resolution module

Alberto Diaspro, Simonluca Piazza, Marco Castello and Takahiro Deguchi, IIT, Genova, Italy

 

 

Meetings&CoursesUnit 26/09/2018