Research Groups

Oscar Burrone

Molecular Immunology

Group Leader

Research Interests and Description
Group Members

Oscar Burrone

International Centre for Genetic Engineering and Biotechnology
Padriciano 99
34149 Trieste, Italy

E-mail: burroneicgeb.org
Office tel: +39-040-3757314
Lab tel: +39-040-3757310/311
Office fax: +39-040-226555

Education

Faculty of Sciences, University of Buenos Aires, Argentina, Bachelor in Chemical Sciences, 1975
Faculty of Sciences, University of Buenos Aires, Argentina, PhD in Biological Chemistry, 1979

Career History

Since 1997, Co-ordinator of the PhD Course in Molecular Genetics of the ICGEB, Trieste, Italy.
Since 1990, Senior Scientist and Group Leader of the Molecular Immunology Group of the ICGEB, Trieste, Italy.
1985-1993, Assistant Professor, Instituto de Investigaciones Bioquímicas, Faculty of Sciences, University of Buenos Aires, Argentina.
1985-1989, Member of the Executive Committee of the National Program of Biotechnology, Ministry of Science and Technology, Buenos Aires, Argentina.
1983-1992, Scientific Career Member of the National Research Council (CONICET), Argentina.
1983-1990, Head of the Laboratory of Molecular Immunology and Virology, "Instituto Luis F. Leloir de Investigaciones Bioquimicas", "Fundación Campomar", Buenos Aires, Argentina.
1981-1983, Staff Research Scientist, MRC Laboratory of Molecular Biology, Division of Protein and Nucleic Acid Chemistry, Cambridge, England.
1979-1981, Postdoctoral research fellow, MRC Laboratory of Molecular Biology, Division of Protein and Nucleic Acid Chemistry, Cambridge, England.
1974-1979, Pre-doctoral research fellow, Instituto de Investigaciones Bioquimicas, "Fundación Campomar", and Faculty of Sciences, University of Buenos Aires, Argentina.

Teaching Activity

Since 1998, Lecturer of Biological Sciences, Master in Science Communication, Scuola Internazionale di Studi Superiori Avanzati (ISAS/SISSA), Trieste, Italy.

Scientific Activity

Research interests focus in two major areas: immunology (a) and virology (b). a includes protein engineering of antibody molecules, structural and functional aspects of IgE and its receptors, DNA based vaccines for tumour immunotherapy and receptors of dendritic cells. b includes the molecular biology of rotavirus replication and the role of non-structural proteins in virus morphogenesis.

Selected publications

Arnoldi, F., Burrone, O.R. 2009. Role of viral non-structural proteins in rotavirus replication. Future Virology 4, 185-197

López-Requena, A., Burrone, O.R. 2009. Anti-Idiotypic Antibodies and “Tumor-Only” Antigens: An Update. Open Immun. J. 2, 1-8

Cesco-Gaspere, M., Zentilin, L., Giacca, M., Burrone, O.R. 2008. Boosting anti-idiotype immune response with recombinant AAV enhances tumour protection induced by Gene Gun vaccination. Sc. J. Imm. 68, 58-66 PubMed link


Predonzani, A., Arnoldi, F., López-Requena, A., Burrone, O.R. 2008. In vivo site-specific biotinylation of proteins within the secretory pathway using a single vector system. BMC Biotechnology 8, 41 PubMed link

Pulecio, J., Tagliani, E., Scholer, A., Prete F., Fetler, L., Burrone, O.R., Benvenuti, F. 2008. Expression of Wiskott-Aldrich syndrome protein in dendritic cells regulates synapse formation and activation of naive CD8+ T cells. J.Immunol, 181, 1135-1142

Tagliani E., Guermonprez P., Sepúlveda J., Lopez Bravo M., Ardavin C., Amigorena S., Benvenuti, F., Burrone O.R. 2008. Selection of an antibody library identifies a pathway to induce immunity by targeting CD36 on steady state CD8a+ dendritic cells. J. Immunol. 180, 3201-3209 Pubmed link

Campagna , M., Budini, M., Arnoldi, F., Desselberger, U., Allende, J.E., Burrone, O.R. 2007. Impaired hyperphosphorylation of rotavirus NSP5 in cells depleted of casein kinase 1a is associated with the formation of viroplasms with altered morphology and a moderate decrease in virus replication. J. Gen. Virol., 88, 2800-10 PubMed link

Arnoldi, F., Campagna, M., Eichwald, C., Desselberger, U., Burrone, O.R. 2007. The interaction of the rotavirus polymerase VP1 with the non-structural protein NSP5 is stronger than with NSP2. J. Virol. 81, 2128-2137

Bestagno, M., Sola, I., Dallegno, E., Sabella, P., Poggianella, M., Plana-Durán, J., Enjuanes, L., Burrone, O.R. 2007. Recombinant dimeric small immune proteins (SIPs) efficiently neutralise Transmissible Gastroenteritis Virus infectivity in vitro and confer passive immunity in vivo. J. Gen. Virol. 88, 187-195

Di Niro R., Ziller, F., Florian, F., Crovella, S., Stebel, M., Bestagno, M., Burrone, O.R. Bradbury, A.R.M., Secco, P., Marzari, R., Sblattero, D. 2007. Construction of miniantibodies for the in vivo study of human autoimmune diseases in animal models. BMC Biotechnology 7, 46

López-Requena, A., Bestagno, M., Mateo de Acosta, C., Cesco-Gaspere, M., Vázquez, A.M., Pérez, R., Burrone, O.R. 2007. Gangliosides, Ab1 and Ab2 antibodies. III. The idiotype of anti-ganglioside mAb P3 is immunogenic in a T cell-dependent manner. Mol. Immunol. 44, 2915-2922

López-Requena, A., Mateo de Acosta, C., Bestagno, M., Vázquez, A.M., Pérez, R., Burrone, O.R. 2007. Gangliosides, Ab1 and Ab2 antibodies. IV. Dominance of VH domain in the induction of anti-idiotypic antibodies by gene gun immunisation. Mol. Immunol. 44, 3070-3075

Alamillo, J.M., Monger, W., Solá. I., Garcia, B., Perrin, Y., Bestagno, M., Burrone, OR., Plana-Duran, J., Enjuanes, L., Lomonossoff, GP., Garcia, JA. 2006. Use of virus vectors for the expression in plants of active full-length and single chain anti-coronavirus antibodies. Biotechnology J. 1, 1103-1111

Campagna, M., Burrone, O.R. 2006. Fusion of Tags induces spurious phosphorylation of rotavirus NSP5. J. Virol. 80, 8283-8285

Monger, W. Alamillo, J.M., Solá. I., Perrin, Y., Bestagno, M., Burrone, O.R., Sabella, P., Plana-Duran, J., Enjuanes, L., Garcia, J.A., Lomonossoff, G.P. 2006. An antibody derivative expressed from viral vectors passively immunises pigs against Transmissible Gastroenteritis Virus infection when supplied orally in crude plant extracts. Plant Biotechnol. J. 4, 623-631

Poggianella, M., Bestagno, M., Burrone, O.R. 2006. The Extracellular Membrane Proximal Domain of human membrane IgE controls apoptotic signalling of the B Cell Receptor in the mature cell line A20. J. Immunol. 177, 3597-3605

Campagna, M., Eichwald, C., Vascotto, F., Burrone, O.R. 2005. RNA interference of rotavirus segment 11 mRNA reveals the essential role of NSP5 in virus replication. J. Gen. Virol. 86, 1483-1489

Cesco-Gaspere, M., Benvenuti F., Burrone, O.R. 2005. BCL1 lymphoma protection induced by idiotype DNA vaccination is entirely dependent on anti-idiotypic antibodies. Cancer Immunol. Immunother. 54, 351-358

Vangelista, L., Soprana, E. Cesco-Gaspere, M., Mandiola-Sáenz, P., Paganelli, G., Burrone, O.R., Antonio G. Siccardi, A.G. 2005. Membrane IgE binds and activates FceRI in an antigen independent manner. J. Immunol. 174, 5602-5611

Eichwald, C., Jacob, G., Muszynski, B., Allende, J.E., Burrone, O.R. 2004. Uncoupling substrate and activation functions of rotavirus NSP5: phosphorylation of Ser67 by CK1 is essential for hyper-phosphorylation. Proc. Natl. Acad. Sci. USA, 101, 16304-16309

Vangelista, L., Cesco-Gaspere, M., Lamba D., Burrone, O.R. 2003. Efficient folding of the FceRI a-chain membrane-proximal domain D2 depends on the presence of the N-terminal domain D1. J. Mol. Biol. 322, 815-825

Sepúlveda, J., Jin, H., Sblattero, D., Bradbury, A., Burrone, O.R. 2003. Binders based on dimerised immunoglobulins VH domains. J. Mol. Biol. 333, 355-365

Cod. Fisc. 90031700322 tl_files/trieste pics/5permille .png

AREA Science Park
Padriciano 99
34149 Trieste, ITALY
Tel: +39-040-37571
Fax: +39-040-226555
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